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GC-MS/ LC-MS

Life Sciences > Technology > GC-MS/ LC-MS

Gas Chromatography/ Mass Spectometry (GC-MS)

CANTEST research and analysis facilities offer a wide range of techniques, including gas chromatography (GC) and gas chromatography/ mass spectrometry (GC-MS). GC-MS combines the selectivity of gas chromatography with the sensitivity of mass spectrometry for detection. Where sensitivity is not a priority, conventional flame ionization detection (FID) may be employed.

These techniques are particularly suitable for characterization, identification and quantitation of volatile and semi-volatile organic compounds with relatively low molecular weights (<400 Daltons). GC-MS can also be used to characterize unknown components in a sample by comparing retention times with the comprehensive NIST database library.

Specific applications include the following:

  • Fatty Acid Analysis (e.g. Stearate)
  • Natural Products Analysis (e.g. oils & fragrances)
  • Unknown Characterization
  • Purity Checks (evaluating effectiveness of purification procedures)

Liquid Chromatography/ Mass Spectrometry (LC-MS)

CANTEST uses other analytical methods such as: high performance liquid chromatography (HPLC) and liquid chromatography/ mass spectrometry (LC-MS). LC-MS is a powerful analytical separation technique that combines the selectivity of chromatography with the sensitivity of MS detection. 

LC-MS vs GC-MS

LC-MS is used to analyze a wider range of compounds than GC-MS. Large, polar and thermally labile analytes that are not amenable to GC may be studied by LC-MS without derivatization. The MS source is capable of operating in both electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) modes; it is both compatible with most chromatographic separations and can monitor both positive and negative ion formations.

Specific applications include the following:

  • Routine drug analysis and selective quantitation at trace levels
  • Separation and analysis of complex compound mixtures
  • Structural interpretation and identity confirmation of a diverse range of organic compounds
  • Molecular weight determination and
  • Purity check of LC peaks employing 3D U/V and visible wavelength monitoring with simultaneous MS detection
     
        
     

 
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